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Hand on Hybridization in Papaya

Floral Biology
Papaya is a polygamous plant three basic types of flowers viz. staminate, pistillate and hermaphrodite (bisexual). Of these, only pistillate is stable, whereas flowers of hermaphrodite and male vary in sex expression under different environmental conditions.

Fig:  Papaya flowers withone petal removed to show internal      parts  (a–c) and inflorescences (d–f).  (a) Staminate flower showing stamens (st), pistillode (pi) and corolla tube (ct). (b) Perfect flower showing st, ct, stigmata (sa), petal (p) and an elongated ovary (o). (c) Pistillate flowers showing sepals (sp), petals and round ovary (o). (d) Long male inflorescence with dozens of staminate flowers. (e) Andromonoecious cyme showing one dominant perfect (pf) and five secondary staminate flowers (sf). (f) Female cyme with three pistillate flowers




Staminate flowers
Staminate flowers serve as pollinators. These plants show 1 to 1.5 m long flower stalks hanging out from trunk. The individual flower is small, tubular and contains stamens only.

Pistillate Flowers
Pistillate flowers are large, yellow, borne singly or in – group of three in the leaf axils close to the trunk. The flowers have fine large twisted and fleshy petals that surround an ovary, which swells and develops into papaya fruit. The fruits developed from pistillate flower are spherical to oblong shaped having thick, yellow to orange  coloured flesh in different cultivars with large to small cavity in which numerous round wrinkled black seeds are attached.

Hermaphrodite or bisexual flowers
It has both male and female organs. Individual flowers are 3.5 to 4.5 cm long with tubular base that widens into goblet shape and then spreads out into 5 thick yellow coloured recurred petals. In between these petals, male organs i.e. stamens are present and female organs containing oblong ovary which develops into cylindrical fruits.
Hybridization: Techniques and Consequences

The mating or crossing of two plants or a line of dissimilar genotype is known as hybridization.  In plants, crossing is done by placing pollen grains from one genotype, the male parent, on to the stigma of flowers of the other genotype, the female parent.  It is essential to prevent self-pollination as well as chance cross-pollination in the flowers of the female parent.  At the same time, it must be ensured that the pollen from desired male parent reaches the stigma of female flowers for successful fertilization.  The seeds as well as the progeny resulting from the hybridization are known as hybrid or F1. 

Intervarietal Hybridization: The parents involved in hybridization belong to the same species; they may be two strains, varieties or races of the same spiciest.  It is also known as intraspecific hybridization.  In crop improvement programmes, inter-varietal hybridizations the most commonly used.  In fact, it is so common that it may often appear to be the only form of hybridization used in crop improvement.  An example would be crossing of two varieties of papaya e.g.  CO 3 (CO 2 x Sunrise solo).
Papaya is a polygamous nature of the fruit plant.  Therefore it is imperative to know the cross combination and its segregation ration in the next generation prior to initiate the crossing prgramme. The cross following cross combination and their segregations are given below.



Hybridization procedure or steps involved in hybridization
Details of the following steps have to be known prior to initiating the any hybridization programme in papaya.
1. Choice or selection of parents:  selection of female and male parents
2. Emasculation: it required normally in those cross combination where hermaphrodite flower used as female parent.
4. Crossing or pollination: collect the pollen grains from desired/selected male plants which dehisced recently. To cross-pollinate, one or 2 stamens from a bisexual flower are placed on the pistil of a female flower about to open and a bag is tied over the flower for a few days. At full opening, the stigma is dusted with pollen from a selected male bloom and the bag quickly resealed and it remains so for 7 days.
5. Bagging: Immediately after emasculation of the flower enclosed with suitable bags of appropriate size to prevent random cross pollination.Bags are tied over bisexual blossoms for several days to assure that they are self-pollinated. Paper bags has to be removed after two weeks of the selfing/crossing to facilitate the fruit growth
6. Tagging: The flowers are tagged just after bagging. They are attached to the flower with the help of thread. The following may be recorded on the tag with pencil or high quality permanent markers.
·         Date of emasculation   (DOE):
·         Date of pollination  (DOP):
·         Parentage: Parents:                     
·         No. of flowers emasculated     
·         Signature

6. Harvesting of fruits for F1 seed
7. Isolation of F1 seed from crossed fruits.
8. Raising F1 generation 
Precautions
· Use needles and forceps carefully otherwise latex will come out from ovary of the pistillate/hermaphrodite flower which will lead flower drop.Do not select any virus infected plants for crossing otherwise there is strong possibilities of the mechanical transfer of the diesess.
·         Do not select first 3-4 flower for the crossing which leads poor quality seed.